In this type of chromatography, retention relies on the attraction amongst solute ions and charged internet sites sure to the stationary phase.What is Mobile Phase: It is a solvent or combination of solvent that does move throughout the stationary phase. Since it constantly flows in the stationary stage, it takes the compounds with it to different
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Reply: Within an HPLC procedure, guard columns are placed in between the injector plus the analytical column, largely to shield the analytical column.He then poured more solvent into the column right until the samples ended up eluted at The underside in the column. The results of this method most crucial to his investigation was the plant pigments